Evaluation of two commercial media used in semen cryopreservation on the motility and viability of reparo bulls.
Abstract
Jaripeo bulls have become very important nowadays in Mexico because of their cultural value and their genetic material must be preserved. Therefore, the application of reproductive biotechnologies such as semen cryopreservation is a tool used in bovines that allows maintaining the genetic material viable indefinitely. In the present work, two commercial media that have not been tested in jaripeo bulls that do not have a defined breed were used to evaluate the motility and viability of spermatozoa after the cryopreservation process, which allowed comparing both commercial media in a conventional freeze-thaw protocol in reparo bulls. The results obtained show a higher post-thaw viability of the spermatozoa diluted with Triladyl® 58.44% with respect to the AndroMed® medium 47.72%, however, in terms of post-thaw motility of the spermatozoa there was no significant statistical difference between both media.
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https://revista.asebir.com/preparacion-del-semen-para-tecnicas-de-reproduccion-asistida/
Argudo, D., D. Galarza, P. Bueno, C. Iñiguez, S. Méndez, M. Soria, C. Torres, F. Perea, R. Alberio. 2019. Methods of collection, extender type, and freezability of semen collected from creole bulls raised in the tropical highlands of Ecuador. Trop. Anim. Health. Prod. 51(7): 1839-1845.
Caraballo Guerrero Daniel M., et.al. Comparación de dos diluyentes para criopreservar semen de bovino bajo condiciones de campo en el trópico húmedo. Ciencia animal [Internet]. 2009 [citado 06 mayo 2022]:2. Disponible en: https://www.uv.mx/veracruz/cienciaanimal/files/2013/11/Comparacion-de-dos-diluyentes-comerciales-para-preservar-semen-bovino.pdf
Galarza, A. 2013. Eficacia de dos diluyentes: tris + lecitina de soya (Andromed®) y tris + yema de huevo (Triladyl ®), en la crioconservación de semen de toro de la raza Jersey en Cuenca-Ecuador. Tesis de Maestría. Facultad de Ciencias Agropecuarias. Universidad de Cuenca, Ecuador.
Martinez, C. O., Juárez-Mosqueda, M. L., Hernández, J., Valencia, J. 2006. Cryopreservation of bull spermatozoa alters the perinuclear theca. Theriogenology, 66, 1969-1975.
Molano Villamil Diego Alejandro, Lombana Portes Harol Estiben. Fundamentos y métodos para la dilución y congelación de semen en bovinos. Beatstream [Internet]. 2021 [citado 06 mayo2022]:7-8. Disponible en: https://repository.ucc.edu.co/bitstream/20.500.12494/34389/7/2021_fundamentos_m%C3%A9todos_diluci%C3%B3n.pdf
Mostek, A., Dietrich, M.A., Slowinska, M., Ciereszko, A. 2017. Cryopreservation of bull semen is associated with carbonylation of sperm proteins. Theriogenology, 92, 95-102.
Nuñez, A.L., A.A. Rubio. 2015. Comparación de la calidad biológica del semen bovino poscongelado utilizando como crioprotector leche al 2% de grasa, Andromed y Continental one step. Tesis de grado. Escuela Agrícola Panamericana, Zamorano. Honduras.
Pérez Quiroz Luis Alberto. Congelación de semen bovino, utilizando el diluyente triladyl [Tesis de licenciatura]. Cuatitlán Izcalli (Edo. Méx.). Universidad Nacional Autónoma de México. 2008. Disponible en: http://132.248.9.195/ptd2009/junio/0644533/Index.html
Selles, E., Gadea, J., Romar, R., Matas, C. & Ruiz, S. 2003. Analysis of in vitro fertilizing capacity to evaluate the freezing procedures of boar semen and to predict the subsequent fertility. Reprod Domest Anim,38, 66- 72.
Siqueira JB, JD Guimarães, EP Costa, M Henry, CAA Torres, MVGB Silva. 2007. Relação da taxa de gestação com sêmen bovino congelado e testes de avaliação espermática in vivo. Rev Bras Zootec 36, 387-395.
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