Severity of breast myopathies and their relationship with productive, physiological and behavioral welfare indicators in broilers

Miopatías y bienestar de los pollos de engorde


The objective of the experiment was to evaluate the relationship between different degrees of severity of the myopathies known as wooden breast and white stretch marks with physiological, productive and behavioral indicators of well-being. 570 Ross 308 broilers, reared under commercial conditions for 13 weeks, were used. From week 7 to 13, prior to slaughter, the breast of 35 chickens was randomly palpated, according to their consistency and classified as soft, intermediate and firm. Fifteen blood samples were taken from the 35 chickens to obtain the heterophil-lymphocyte ratio, C-reactive protein, and the hematological profile. Group ethograms were performed by direct observation, weekly. Live weight, breast weight, hot carcass weight, and cold carcass weight were recorded. Each breast was classified according to the degree of severity of the lesions of the white streaks and the wooden breast as normal, mild, moderate and severe. The processed breasts were palpated and classified as soft, medium and firm. Each one was measured for force and shear energy. The results showed that the physiological indicators were not related to the severity of the lesions found for both myopathies. Injury severity increased and was significantly associated with age, live weight, and breast weight. Live chicken breast palpation was highly correlated with processed breast palpation, so live palpation can be used as a noninvasive indicator of consistency and severity of lesions in both myopathies. It is required to evaluate more physiological, behavioral and pain indicators in chickens that present white streaks and wooden breast.


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Author Biography

Rebeca María Zamora Sanabria, Universidad de Costa Rica

Profesora e Investigadora Escuela de Zootecnia. Universidad de Costa Rica. Departamento de Avicultura y Fisiología Animal


Alnahhas, N., C. Berri, M. Chabault, P. Chartrin, M. Boulay, M. C. Bourin, and E. le Bihan Duval. 2016. Genetic Parameters of White Striping in Relation to Body Weight, Carcass Composition, and Meat Quality Traits in Two Broiler Lines Divergently Selected for the Ultimate PH of the Pectoralis Major Muscle. BMC Genetics 17 (1).

Amaral, P., C. Zimermann, L. Santos L, M. Noro , M. Prá, and F. Pilotto. 2017. Evaluation of Physiological Parameters of Broilers with Dorsal Cranial Myopathy. Brazilian Journal of Poultry Science. 2017 Mar;19(1):69–74.

Bailey, R.A., K.A.Watson, S.F. Bilgili, and S. Avendano. 2015. The Genetic Basis of Pectoralis Major Myopathies in Modern Broiler Chicken Lines. Poultry Science 94 (12): 2870–79.

Bokkers, E.A.M. 2004. Behavioural Motivations and Abilities in Broilers. PhD Thesis. University of Wageningen. Wageningen. Netherlands.

Bozakova, N., K. Stoyanchev, D. Girginov, and T. Stoyanchev 2007. Ethological Study of Broiler Chickens after Induction and Treatment of Muscular Dystrophy. Trakia Journal Science; 5 (3):5.

Cavitt, L.C., G. W. Youm, J. F. Meullenet, C. M. Owens, and R. Xiong. 2004. Prediction of Poultry Meat Tenderness Using Razor Blade Shear, Allo-Kramer Shear, and Sarcomere Length. Journal of Food Science 69 (1): SNQ11–15.

Chatterjee, D., H. Zhuang, B. C. Bowker, A. M. Rincon, and G. Sanchez-Brambila. 2016. Instrumental Texture Characteristics of Broiler Pectoralis Major with the Wooden Breast Condition. Poultry Science 95 (10): 2449–54.

Costa, L.S., D.F Pereira, L.G.F Bueno, and H. Pandorfi. 2012. Some Aspects of Chicken Behavior and Welfare. Brazilian Journal of Poultry Science 14 (3): 159–64.

Cotter, P.F. 2015. An Examination of the Utility of Heterophil-Lymphocyte Ratios in Assessing Stress of Caged Hens. Poultry Science 94 (3): 512–17.

Davis, A.K., D.L. Maney, and J.C. Maerz. 2008. The use of leukocyte profiles to measure stress in vertebrates: a review for ecologists. Functional Ecology; 22 (5):760–72.

Dawkins, M. S. 1999. The Role of Behaviour in the Assessment of Poultry Welfare. Worlds Poultry Science Journal 55 (3): 302–3.

De Almeida Mallmann, B., 2019. Prediction and Evaluation of Breast Myopathy. PhD Thesis. University of Arkansas. Arkansas. USA.

Eckersall, P.D., and Bell R. 2010. Acute Phase Proteins: Biomarkers of Infection and Inflammation in Veterinary Medicine. Veterinary Journal 185 (1): 23–27.

Gentle, M.J. 2011. Pain issues in poultry. Applied Animal Behavior Science. Dec 15;135(3):252–8.

Griffin, J.R., L. Moraes, M. Wick M, and M.S. Lilburn. 2018. Onset of White Striping and Progression into Wooden Breast as Defined by Myopathic Changes Underlying Pectoralis Major Growth. Estimation of Growth Parameters as Predictors for Stage of Myopathy Progression. Avian Pathology: Journal of the W.V.P.A 47 (1): 2–13.

Gross, W.B., and S. Siegel. 1983. Evaluation of the Heterophil/Lymphocyte Ratio as a Measure of Stress in Chickens. Avian Diseases 27 (4): 972–79.

Harvey, J W. 2001. Atlas of Veterinary Hematology. Edited by J W Harvey. Atlas of Veterinary Hematology: Blood and Bone Marrow of Domestic Animalsr. Philadelphia: W.B. Saunders. USA. 44 p. ISBN 9780721663340.

Huang X., and D.U. Ahn. 2018. The Incidence of Muscle Abnormalities in Broiler Breast Meat: A Review. Korean Journal of Food Science Animal Resources. 2018 Oct;38(5):835–50.

Kuttappan, V.A. Brewer V.B, Apple J.K, Waldroup P.W, and Owens C.M. 2012. Influence of Growth Rate on the Occurrence of White Striping in Broiler Breast Fillets. Poultry Science 91 (10): 2677–85.

Kuttappan, V.A., V.B. Brewer, A. Mauromoustakos, S.R. McKee, J.L. Emmert, J.F. Meullenet, and C.M. Owens. 2013a. Estimation of Factors Associated with the Occurrence of White Striping in Broiler Breast Fillets. Poultry Science 92 (3): 811–19.

Kuttappan, V.A., Huff G.R, Huff W.E, Hargis B.M, Apple J.K, and C. Coon, 2013b. Comparison of Hematologic and Serologic Profiles of Broiler Birds with Normal and Severe Degrees of White Striping in Breast Fillets. Poultry Sciences. 2013 Feb 1;92(2):339–45. 10.3382/PS.2012-02647.

Kuttappan, V.A., B.M. Hargis, and C.M. Owens. 2016. White Striping and Woody Breast Myopathies in the Modern Poultry Industry: A Review. Poultry Science 95 (11): 2724–33.

Lee, Y.S., C.M. Owens, C. Mand, and J. F. Meullenet. 2008. The Meullenet-Owens Razor Shear (Mors) for Predicting Poultry Meat Tenderness: Its Applications and Optimization. Journal of Texture Studies 39 (6): 655–72.

Lorenzi, M., S. Mudalal, C. Cavani, and M. Petracci. 2014. Incidence of White Striping under Commercial Conditions in Medium and Heavy Broiler Chickens in Italy. Journal of Applied Poultry Research 23 (4): 754–58.

MacRae, V.E., M. Mahon, S. Gilpin, D.A. Sandercock, and M.A. Mitchell. 2006. Skeletal Muscle Fibre Growth and Growth Associated Myopathy in the Domestic Chicken (Gallus Domesticus). British Poultry Science 47 (3): 264–72.

Manteca, X. 2016. Use of Outcome Based Measures and Design Based Measures. In 4th OIE Global Conference of Animal Welfare, edited by OIE. Guadalajara, México: OIE.

Marques, T. A. 2016. Molecular Biomarkers of Welfare in Poultry. Proceeding of Veterinary and Animal Science Days, Vol 2, No 1s. doi:

Mellor, D.J. 2017. Operational Details of the Five Domains Model and Its Key Applications to the Assessment and Management of Animal Welfare. Animals Aug 7(8): 60.

Norring, M., E. Kaukonen, and A. Valros. 2016. The Use of Perches and Platforms by Broiler Chickens. Applied Animal Behaviour Science 184. November. 91-96.

Petracci, M., S. Mudalal, F. Soglia, and C. Cavani. 2019. Meat Quality in Fast-Growing Broiler Chickens. Worlds Poultry Science Journal Vol 71 (2): 363–74.

Scanes, C.G. 2016. Biology of Stress in Poultry with Emphasis on Glucocorticoids and the Heterophil to Lymphocyte Ratio. Poultry Science. Sep 1;95 (9): 2208–15.

Shini, S., P. Kaiser, A. Shini A, and W. L Bryden. 2008. Differential Alterations in Ultrastructural Morphology of Chicken Heterophils and Lymphocytes Induced by Corticosterone and Lipopolysaccharide. Veterinary Immunology and Immunopathology 122 (1–2): 83–93.

Shields, S.J., J.P Garner, and J.A Mench. 2005. Effect of Sand and Wood-Shavings Bedding on the Behavior of Broiler Chickens. Poultry Science 84 (12): 1816–24.

Sohail M.U., A. Ijaz, M.S. Yousaf, K. Ashraf, H. Zaneb, and M. Aleem. 2010. Alleviation of Cyclic Heat Stress in Broilers by Dietary Supplementation of Mannan-oligosaccharide and Lactobacillus-based probiotic: Dynamics of Cortisol, Thyroid hormones, Cholesterol, C-reactive protein, and Humoral Immunity. Poultry Science. 2010 Sep; 89 (9):1934–8.

The Jamovi Project. 2019. Jamovi. (Version 1.0) [Computer Software]. Retrieved from

Tijare, V., F. L. Yang, V. A. Kuttappan, C. Z. Alvarado, C. N. Coon, and C. M. Owens. 2016. Meat Quality of Broiler Breast Fillets with White Striping and Woody Breast Muscle Myopathies. Poultry Science 95 (9): 2167–73.

How to Cite
Zamora Sanabria, Rebeca María, Alma Vásquez-Delgado, Jorge Elizondo-Salazar, Jorge Camacho-Sandoval, and María del Pilar Castañeda-Serrano. 2022. “Severity of Breast Myopathies and Their Relationship With Productive, Physiological and Behavioral Welfare Indicators in Broilers: Miopatías Y Bienestar De Los Pollos De Engorde”. Latin American Archives of Animal Production 30 (3), 239-51.
Original paper