Degradation kinetics and ruminal digestibility of two protein sources evaluated by in vitro and in situ methods
Abstract
This study compared the bromatological values, in vitro degradation, and in situ digestibility of coconut (Cocos nucifera L.) and cacahuananche (Gliricidia sepium L.) pastes to assess their biological values and potential relevance for in vivo testing. The study was conducted at the Animal Nutrition Laboratory, Faculty of Veterinary Medicine and Zootechnics at the Autonomous University of Guerrero, Mexico. Samples were analyzed in a completely randomized design using InfoStat software, with mean differences determined by Tukey’s test (P < 0.05). Bromatological analysis showed that both pastes had similar contents of dry matter, ash, and organic matter (P > 0.05). cacahuananche paste had 159 % more crude protein, while coconut paste contained 151 % more neutral detergent fiber and 136% more acid detergent fiber (P < 0.05). In the in vitro assay, cacahuanache paste exhibited higher dry matter degradability at 12, 24, and 48 hours, whereas coconut paste showed higher degradation at 72 hours (P < 0.05). In situ digestibility kinetics indicated that cacahuananche paste had significantly higher fractions and a positive correlation compared to coconut paste (P < 0.05). In conclusion, cacahuananche paste, compared to coconut paste, presents a promising alternative protein source for ruminants, warranting further in vivo studies to confirm its effectiveness in formulated diets.
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