Effect of inoculum (rumen fluid or faecal inoculum) on in vitro gas production in feedstuffs for sheep
Abstract
An experiment was carried out with the objective of evaluating the effect of two types of inocula from Saint Croix sheep (T1: ruminal fluid, T2: excreta with yeast), in two types of feed with different proportions of concentrate (C) and forage (F), (75C:25F; 25C:75F), on gas production at 24, 36 and 48 h of incubation (in duplicate) in 100 mL syringes (n = 3 replicates per diet), in order to determine in a factorial arrangement 2 X 2, (with two inocula and two diets), if the excreta can replace ruminal fluid as inoculum in in vitro gas production. Gas production was higher with ruminal fluid than with excreta, and with the high-concentrate diet. In conclusion, the excreta inoculum with yeast can be used to estimate the gas production obtained with ruminal fluid in in vitro incubations of feed for sheep.
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References
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