Genetic-biochemical polymorphism of enzymes in Brazilian Mangalarga mares

Abstract

A study was conducted with a group of mares of the Mangalarga breed in Brazil, with the aim of determining the electrophoretic pattern, in polyacrylmide gel, of the enzymes Acid Phosphatase, Alkaline Phosphatase and Peroxidase. Blood plasma samples of 45 mares in reproductive age were used. For Acid Phosphatase, we observed the existence of two alleles, A and B, with allelic frequencies of 0.28 and 0.72, respectively: corresponding to three genotypes AA, AB and BB. Allelic frequency demonstrated that the group was in Hardy-Weinberg-Castle balance. For Alkaline Phosphatase, three genotypes were verified: Type I (HA1), presenting only one band of fast migration; Type II (HA1-HA2 and HA1-HA3), presenting two bands, with HA2 of intermediary position and HA3 of slower motility; and Type III, presenting all three bands HA1, HA2 and HA3. Type I was most frequent, occurring in 21 animals. For Peroxidase, all animals presented a pattern of two bands, designated as genotype I, suggesting that this is a monomorphic locus in the population under study.