G1 mutation of GDF9 gene and its relationship with birth weight in Pelibuey sheep

Keywords: Pelibuey lambs., Exon 1., Litter size., SNP marker., Proificacy.

Abstract

Single nucleotide polymorphisms (SNP) in the GDF9 (Growth Differentiation Factor 9) gene have been associated with litter size or type of birth and birth weight in sheep. The objective of this study was to identify the presence of SNP G1 in exon 1 of the GDF9 gene to relate it to birth weight (BW) and productive variables (weaning weight: WW and daily weight gain: DWG) in breed lambs. Pelibuey. A total of 64 lambs from single (n=9) and multiple (n=55) births were used. Using PCR-RFLP markers, the presence of SNP G1 was identified; with a genotype frequency of 0.84 for the wild type GG and 0.15 for the heterozygous GA genotype. The allele frequency was 0.92 for the (G) allele and 0.078 for the A allele. The AA mutated genotype was not detected. The GA genotype was present in multiple birth lambs. The variables BW, WW and DWG were higher (P<0.05) in lambs from single birth with genotype GG (GGS) than in lambs from multiple birth with genotype GG (GGM) and lambs from multiple birth with GA genotype (GAM). The GGS lambs had a better preweaning performance with respect to the GA and GG genotype lambs from multiple births. Finally, BW, WW and DWG were statistically different between type of birth and type of genotype (P<0.05). This is the first report of this G1 SNP in Pelibuey sheep.

Downloads

Download data is not yet available.

Author Biographies

Juan Salazar-Ortiz, Colegio de Postgraduados Campus Córdoba

Profesor Investigador 

Josafhat Salinas Ruiz, Colegio de Postgraduados Campus Córdoba

Profesor Investigador

Jaime Gallegos-Sánchez, Colegio de Postgraduados Campus Montecillo

Profesor Investigador

Martha Herández-Rodríguez, Colegio de Postgraduados Campus Montecillo

Profesora Investigadora

References

Al-Khuzai, FLJ., & JR Ahmed. 2019. Polymorphism of GDF9 (exon-1) gene and its association with milk production and prolificacy of Awassi sheep. Plant Archives, 19(2), 4037-4040.

CP. 2016. Reglamento para el uso y cuidado de animales destinados a la investigación en el Colegio de Postgraduados. Consejo general académico. ACUERDO 02.11.16.

García, E. (2004). Modificación al sistema de clasificación climática de koppen (5.a ed.). México, D.F.: UNAM.

Getmantseva, L., N Bakoev, S Bakoev, N Shirokova, M Kolosova, A Kolosov y V Shevtsova. 2019. Effect of the GDF9 gene on the weight of lambs at birth. Bulgarian Journal of Agricultural Science, 25(1), 153-157.

Hanrahan, J. P., SM Gregan, P Mulsant, M Mullen, GH Davis, R Powell y SM Galloway. 2004. Mutations in the genes for oocyte-derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in Cambridge and Belclare sheep (Ovis aries). Biology of reproduction, 70(4), 900-909.

Hernández, V. R., VV Murillo, RG Costa, VL Parraguirre, MDLA Valencia de Ita, y O Romero-Arenas. 2022. Evaluation of Genetic Parameters of Growth of Pelibuey and Blackbelly Sheep through Pedigree in Mexico. Animals, 12(6), 691.

Hinojosa-Cuéllar, J. A., J Oliva-Hernández, G Torres-Hernández, JC Segura-Correa, EM Aranda-lbáñez, y JM González-Camacho. 2012. Factores que afectan el crecimiento predestete de corderos Pelibuey en el trópico húmedo de México. Universidad y ciencia, 28(2), 163-171.

Kirikci, K., MA Cam, y L Mercan. 2021. Investigation of G1 (c. 260G> A) polymorphism in exon 1 of GDF9 gene in Turkish sheep breed Karayaka. Turkish Journal of Veterinary & Animal Sciences, 45(1), 191-197.

Mohammad, M. K., HH Seyed, y H Nosratollah. 2011. Genetic polymorphism BMP15 and GDF9 genes in Sangsari sheep of Iran. International Journal of Genetics and Molecular Biology, 3(1), 31-34.

Muñoz-García, C., H Vaquera-Huerta., J Gallegos-Sánchez., CM Becerril-Pérez., LA Tarango-Arámbula., Á Bravo-Vinaja., y Cortez-Romero. 2021. Influence of FecGE mutation on the reproductive variables of Pelibuey ewes in the anestrus period. Tropical Animal Health and Production, 53(2), 328.

Noriega Loyo, J. 2017. Determinación del efecto del polimorfismo del gen leptina en el desarrollo corporal-calidad de la canal de corderos Pelibuey (Master's thesis). Colegio de Postgraduados.

Paz, E., J Quinones., S Bravo., E Rodero., A Gonzalez., y N Sepúlveda, N. 2014. Identification of G1 and G8 polymorphisms of GDF9 gene in Araucano creole sheep. Archivos de Medicina Veterinaria, 46(2), 327-331.

Pérez‐Ruiz, E., J Gallegos‐Sánchez, C Cortez‐Romero, OL Segura‐León, J Salinas‐Ruíz y J Salazar‐Ortiz. 2020. FecGE mutation in Pelibuey sheep. Animal Genetics, 51(2), 346-347.

Silva, B. D. M., A Castro, E., CD Souza, R Paiva S., R Sartori., M Franco, M y DO Melo, E. 2011. A new polymorphism in the Growth and Differentiation Factor 9 (GDF9) gene is associated with increased ovulation rate and prolificacy in homozygous sheep. Animal genetics, 42(1), 89-92.

Souza, C. J. H., AS McNeilly., VM Benavides., EO Melo y CF Moraes, J. 2014. Mutation in the protease cleavage site of GDF9 increases ovulation rate and litter size in heterozygous ewes and causes infertility in homozygous ewes. Animal Genetics, 45(5), 732–739.

Tong, B., J Wang, Z Cheng, J Liu, Y Wu, Y Li y G Li. 2020. Novel variants in GDF9 gene affect promoter activity and litter size in Mongolia sheep. Genes, 11(4), 375.

Våge, D. I., M Husdal., MP Kent., G Klemetsdal y IA Boman. 2013. A missense mutation in growth differentiation factor 9 (GDF9) is strongly associated with litter size in sheep. BMC genetics, 14, 1-8.

Wang, F., M Chu, L Pan, X Wang, X He, R Zhang y R Di. 2021. Polymorphism detection of GDF9 gene and its association with litter size in Luzhong mutton sheep (Ovis aries). Animals, 11(2), 57.

Published
2023-09-26
How to Cite
Rosas-Rodríguez, Miriam, Juan Salazar-Ortiz, Josafhat Salinas Ruiz, Jaime Gallegos-Sánchez, Martha Herández-Rodríguez, and Cesar Cortez-Romero. 2023. “G1 Mutation of GDF9 Gene and Its Relationship With Birth Weight in Pelibuey Sheep”. Archivos Latinoamericanos De Producción Animal 31 (3), 243-49. https://doi.org/10.53588/alpa.310303.
Section
Original paper