Effect of reduced glutathione (GSH) on the sperm quality of verraco under subhumid tropic conditions
Keywords:
GSH, preserved semen, boar
Abstract
In this work was evaluate the effect of reduced glutathione (GSH) on sperm quality of boars under tropic conditions. 40 ejaculates from 4 boars (10 replicates per boar) of commercial lines Yorkshire / Landrace and Pietrain / Duroc between 24 and 28 months of age, were collected at 7 day intervals by the gloved hand technique.The ejaculate was diluted 1:10 with 2000 x106 (viable sperm), L-Reduced Glutathione (GSH, Sigma-Aldrich ®) added to the diluent half conservation (MIII ®). Three groups were formed, control group without GSH (0GSH) and two treatments with GSH concentrations 1 mM (1GSH) and 2 mM (2GSH), total mobility, integrity of the plasma membrane, osmotic resistance and acrosomal integrity initial and in the semen conserved at 17º C was evaluated at 0, 24, 48, 72 and 96 h. The plasma membrane integrity of boar sperm supplemented with 2GSH was 88.0% and 1GSH of 87.3%, different with respect 0GSH (P <0.05). In total mobility, in 2GSH and 1GSH was 58.9% and 56.9%, respectively, showing differences (P <0.05) with respect to the 0GSH (52.0%). During the conservation period from 72 and to 96 h of storage, total mobility showed a 14% decrease for 0GSH, while that in IGSH nad 2GSH only a decrease of 11 and 6% respectively, was observed. Plasma membrane integrity at 96 h was 87.52%, 85.40% and 80.65% for 2GSH, 1GSH and 0GSH, respectively (P<0.05). The variables osmotic tolerance and acrosomal integrity not show significant differences (P> 0.05) in between treatments during the time of conservation. The supplementation with 2 mM of GSH to the dilution medium improvement the quality of spermatozoa of boars maintained in tropical conditions conserved at 17° C
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References
Ariagno, J., E. Mormandi. 2016. Guía práctica para la evaluación del semen. Guía Práctica ByPC, 80(3):29-36.
Bucak, MN, P.B. Tuncer, S. Sariôzkan, N. Baspinar, M. Taspinar, K. Coyan, A. Bilgili, P.P. Akalin, S. Bûyûkleblebici, S. Aydos, S. Ilgaz, A. Sunguroglu, and D. Oztuna. 2010. Effect of antioxidants on post-thawed bovine sperm and oxidative stress parameters: antioxidants protect DNA integrity against cryodamage. Cryobiology, 61 (3):248-253. https://doi.org/10.1016/j.cryobiol.2010.09.001
Buonocore, G, S. Perrone, and M.L Tataranno. 2010. Oxygen toxicity: chemistry and biology of reactiveoxygen species. Seminars in Fetal & Neonatal Medicine, 15:186-190. https://doi.org/10.1016/j.siny.2010.04.003
Confederación de Porcicultores Mexicanos A. C. 2018. Compendio estadístico del sector porcícola 2018.
Córdova, J.C.A. 2010. Control de la peroxidación lipídica de semen refrigerado y criopreservado de verraco mediante antioxidantes (α-Tocoferol / Glutatión reducido), y su repercusión sobre la calidad espermática. Tesis Doctoral. Facultad de Veterinaria, Departamento de sanidad animal. Universidad de León. León, España. https://dialnet.unirioja.es/servlet/tesis?codigo=106440
Estrada, P.E. 2014. Efectos del glutatión reducido y la procaína en la resistencia a la criopreservación de semen porcino. Acciones a nivel de la estabilidad nuclear y su efecto en la fertilidad “in vivo”. Tesis Doctoral. Universitat Autonoma de Barcelona. Barcelona, España. https://ddd.uab.cat/record/127114
González-Peña D., R.V. Knox, J. Pettigrew, and S.L. Rodríguez-Zas. 2014. Impact of pig insemination technique and semen preparation on profitability. Journal of Animal Science, 92:72-84. https://doi.org/10.2527/jas.2013-6836
Henning, H, T.T. Ngo, and D. Waberski. 2015. Centrifugation stress reduces the responsiveness of spermatozoa to a capacitation stimulus in in vitro-aged semen. Andrology, 3: 834-842. https://doi.org/10.1111/andr.12064
Hicks, J.J., R.Y. Torres, and V.M. Sierra. 2006. Estrés oxidativo. Concepto y clasificación. Endocrinología y Nutrición, 4:223-226. https://www.medigraphic.com/cgi-bin/new/resumen.cgi?IDARTICULO=11555
INEGI, 2019. Encuesta Nacional Agropecuaria. Disponible en: www.inegi.org.mx/programas/ena/default.html#Tabulados
Li, H., X.G. Zhang, Q. Fang, Q. Liu, R.R. Du, G.S. Yang, L.Q. Wang. and J.H. Hu. 2017. Supplemental effect of different levels of taurine in Modena on boar semen quality during liquid preservation at 17°C. Animal Science Journal, 88(11): 1692-1699. https://doi.org/10.1111/asj.12865
Little, R.C., P.R. Henry, and C.B. Ammerman. 1998. Statistical analysis of repeated measures data using SAS procedures. Journal Animal Science, 76:1216-1231. https://doi.org/10.2527/1998.7641216x
Mata-Campuzano, M., M. Alvarez-Rodriguez, E. Del Olmo, M.R. Fernández-Santos, J.J. Garde, and F. Martínez-Pastor. 2012. Quality, oxidative markers and DNA damage (DNA) fragmentation of red deer thawed spermatozoa after incubation at 37°C in presence of several antioxidants. Theriogenology, 78:1005-1019. https://doi.org/10.1016/j.theriogenology.2011.12.018
Membrillo, O.A., I.A. Córdova, G.J.J. Hicks, C.I.M. Olivares, T.V.M. Martínez, y M.J.J. Valencia. 2003. Peroxidación lipídica y antioxidantes en la preservación de semen. Una revisión. Interciencia, 28(12):699-704. http://ve.scielo.org/scielo.php?script=sci_arttext&pid=S0378-18442003001200006
Obando, P., M. Alfaro, E. Hurtado, y T. Rodríguez. 2012. Respuesta reproductiva de cerdas multíparas a la adición de oxitocina y prostaglandina F2 alfa previo a la inseminación artificial. Zootecnia Tropical, 30(2):169-174.http://ve.scielo.org/scielo.php?script=sci_arttext&pid=S0798-72692012000200005&lng=es&tlng=es
Peruma, P., J.K. Chamuah, and C. Rajkhowa. 2013. Effect of catalase on the liquid storage of mithun (Bos frontalis) semen. Asian Pacific Journal of Reproduction, 2(3): 209-214. https://doi.org/10.1016/S2305-0500(13)60148-7
Petruška, P., M. Capcarova, and P. Sutovsky. 2014. Antioxidant supplementation and purification of semen for improved artificial insemination in livestock species. Turkish Journal of Veterinary & Animal Sciences, 38:643-652. https://doi.org/10.3906/vet-1404-61
Riesenbeck, A., M. Schulze, K. Rüdiger, H. Henning, and D. Waberski. 2015. Quality control of boar sperm processing: implications from European AI centers and two spermatology reference laboratories. Reproduction Domestic Animals, 50:1-4. https://doi.org/10.1111/rda.12573
Schilling, E., M. Vengust, and M.O. Bajt. 1986.The osmotic resistance (ORT) of boar spermatozoa and the relation to pregnancy rate and litter size. Proc. 9th IPVS Congress. Barcelona. 77.
Schulze, M., C. Ammon, J. Schaefer, A.M. Luther, M. Jun, and D. Waberski. 2017. Impact of different dilution techniques on boar sperm quality and sperm distribution of the extended ejaculate. Animal Reproduction Science, 182:138-145. https://doi.org/10.1016/j.anireprosci.2017.05.013
SIAP, 2018. Servicio de Información Agroalimentaria y Pesquera, estadísticas 2018. Disponible en: http://infosiap.siap.gob.mx/gobmx/datosAbiertos.php
Trujillo, O.M.E., O.A.J. Contreras, H.S. Espinosa, P. O. Gutiérrez, T.E. Hernández, N.J. Nava, G.R.G. Martínez, R.R. Martínez, C.J.C. Rojas, H.M. Sánchez, V.R.M. Vigueras, y S.G.Y. Castellanos. 2017. El Verraco. 1ª ed. ED. Universidad Nacional Autónoma de México, Facultad de Medicina Veterinaria y Zootecnia, CU, CDMX.
Villa, N.A., L.E. Sánchez, y A. Ceballos. 2008. Actividad de glutatión peroxidasa y superóxido dismutasa en plasma seminal y sangre en cerdos reproductores. Veterinaria y Zootecnia. 3(1). https://doctoradoagrarias.files.wordpress.com/2015/04/vetzootec-2009.pdf
Vongpralub, T., P. Thananurak, C. Sittikasamkit, P. Chuawongboon, M. Duangjinda, W. Boonkum, and V. Chankitisakul. 2016. Comparison of effects of different antioxidants supplemented to long-term extender on boar semen quality following storage at 17ºC. The Thai Journal of Veterinary Medicine, 46(1):119-126. https://he01.tci-thaijo.org/index.php/tjvm/article/view/49791
Vyt, P., D. Maes, C. Quinten, T. Rijsselaere, W. Deley, M. Aarts, A. De Kruif, and A. Van Soom . 2008. Detailed motility evaluation of boar semen and its predictive value for reproductive performance in sows. Vlaams Dierge Tijds, 77:291-298. https://biblio.ugent.be/publication/746864
Yeste, M., M. Briz, E. Pinart, S. Sancho, E. Bussalleu, and S. Bonet 2010. The osmotic tolerance of boar spermatozoa and its usefulness as sperm quality parameter. Animal Reproduction Science, 119:265-274. https://doi.org/10.1016/j.anireprosci.2010.02.011
Yeste, M., E. Estrada, E. Pinart, S. Bonet, J. Miró, and J. E. Rodríguez-Gil. 2014. The improving effect of reduced glutathione on boar sperm cryotolerance is related with the intrinsic ejaculate freezability. Cryobiology, 68(2):251-261. https://doi.org/10.1016/j.cryobiol.2014.02.004
Yeste M. 2017. State of the art of boar sperm preservation in liquid and frozen state. Animal Reproduction, 14(1):69-81. http://dx.doi.org/10.21451/1984-3143-AR895
Zhang X.G., Q. Liu, L.Q. Wang, G.S. Yang, and J.H. Hu. 2016. Effects of glutathione on sperm quality during liquid storage in boars. Animal Science Journal, 87, 1195-1201. https://doi.org/10.1111/asj.12545
Bucak, MN, P.B. Tuncer, S. Sariôzkan, N. Baspinar, M. Taspinar, K. Coyan, A. Bilgili, P.P. Akalin, S. Bûyûkleblebici, S. Aydos, S. Ilgaz, A. Sunguroglu, and D. Oztuna. 2010. Effect of antioxidants on post-thawed bovine sperm and oxidative stress parameters: antioxidants protect DNA integrity against cryodamage. Cryobiology, 61 (3):248-253. https://doi.org/10.1016/j.cryobiol.2010.09.001
Buonocore, G, S. Perrone, and M.L Tataranno. 2010. Oxygen toxicity: chemistry and biology of reactiveoxygen species. Seminars in Fetal & Neonatal Medicine, 15:186-190. https://doi.org/10.1016/j.siny.2010.04.003
Confederación de Porcicultores Mexicanos A. C. 2018. Compendio estadístico del sector porcícola 2018.
Córdova, J.C.A. 2010. Control de la peroxidación lipídica de semen refrigerado y criopreservado de verraco mediante antioxidantes (α-Tocoferol / Glutatión reducido), y su repercusión sobre la calidad espermática. Tesis Doctoral. Facultad de Veterinaria, Departamento de sanidad animal. Universidad de León. León, España. https://dialnet.unirioja.es/servlet/tesis?codigo=106440
Estrada, P.E. 2014. Efectos del glutatión reducido y la procaína en la resistencia a la criopreservación de semen porcino. Acciones a nivel de la estabilidad nuclear y su efecto en la fertilidad “in vivo”. Tesis Doctoral. Universitat Autonoma de Barcelona. Barcelona, España. https://ddd.uab.cat/record/127114
González-Peña D., R.V. Knox, J. Pettigrew, and S.L. Rodríguez-Zas. 2014. Impact of pig insemination technique and semen preparation on profitability. Journal of Animal Science, 92:72-84. https://doi.org/10.2527/jas.2013-6836
Henning, H, T.T. Ngo, and D. Waberski. 2015. Centrifugation stress reduces the responsiveness of spermatozoa to a capacitation stimulus in in vitro-aged semen. Andrology, 3: 834-842. https://doi.org/10.1111/andr.12064
Hicks, J.J., R.Y. Torres, and V.M. Sierra. 2006. Estrés oxidativo. Concepto y clasificación. Endocrinología y Nutrición, 4:223-226. https://www.medigraphic.com/cgi-bin/new/resumen.cgi?IDARTICULO=11555
INEGI, 2019. Encuesta Nacional Agropecuaria. Disponible en: www.inegi.org.mx/programas/ena/default.html#Tabulados
Li, H., X.G. Zhang, Q. Fang, Q. Liu, R.R. Du, G.S. Yang, L.Q. Wang. and J.H. Hu. 2017. Supplemental effect of different levels of taurine in Modena on boar semen quality during liquid preservation at 17°C. Animal Science Journal, 88(11): 1692-1699. https://doi.org/10.1111/asj.12865
Little, R.C., P.R. Henry, and C.B. Ammerman. 1998. Statistical analysis of repeated measures data using SAS procedures. Journal Animal Science, 76:1216-1231. https://doi.org/10.2527/1998.7641216x
Mata-Campuzano, M., M. Alvarez-Rodriguez, E. Del Olmo, M.R. Fernández-Santos, J.J. Garde, and F. Martínez-Pastor. 2012. Quality, oxidative markers and DNA damage (DNA) fragmentation of red deer thawed spermatozoa after incubation at 37°C in presence of several antioxidants. Theriogenology, 78:1005-1019. https://doi.org/10.1016/j.theriogenology.2011.12.018
Membrillo, O.A., I.A. Córdova, G.J.J. Hicks, C.I.M. Olivares, T.V.M. Martínez, y M.J.J. Valencia. 2003. Peroxidación lipídica y antioxidantes en la preservación de semen. Una revisión. Interciencia, 28(12):699-704. http://ve.scielo.org/scielo.php?script=sci_arttext&pid=S0378-18442003001200006
Obando, P., M. Alfaro, E. Hurtado, y T. Rodríguez. 2012. Respuesta reproductiva de cerdas multíparas a la adición de oxitocina y prostaglandina F2 alfa previo a la inseminación artificial. Zootecnia Tropical, 30(2):169-174.http://ve.scielo.org/scielo.php?script=sci_arttext&pid=S0798-72692012000200005&lng=es&tlng=es
Peruma, P., J.K. Chamuah, and C. Rajkhowa. 2013. Effect of catalase on the liquid storage of mithun (Bos frontalis) semen. Asian Pacific Journal of Reproduction, 2(3): 209-214. https://doi.org/10.1016/S2305-0500(13)60148-7
Petruška, P., M. Capcarova, and P. Sutovsky. 2014. Antioxidant supplementation and purification of semen for improved artificial insemination in livestock species. Turkish Journal of Veterinary & Animal Sciences, 38:643-652. https://doi.org/10.3906/vet-1404-61
Riesenbeck, A., M. Schulze, K. Rüdiger, H. Henning, and D. Waberski. 2015. Quality control of boar sperm processing: implications from European AI centers and two spermatology reference laboratories. Reproduction Domestic Animals, 50:1-4. https://doi.org/10.1111/rda.12573
Schilling, E., M. Vengust, and M.O. Bajt. 1986.The osmotic resistance (ORT) of boar spermatozoa and the relation to pregnancy rate and litter size. Proc. 9th IPVS Congress. Barcelona. 77.
Schulze, M., C. Ammon, J. Schaefer, A.M. Luther, M. Jun, and D. Waberski. 2017. Impact of different dilution techniques on boar sperm quality and sperm distribution of the extended ejaculate. Animal Reproduction Science, 182:138-145. https://doi.org/10.1016/j.anireprosci.2017.05.013
SIAP, 2018. Servicio de Información Agroalimentaria y Pesquera, estadísticas 2018. Disponible en: http://infosiap.siap.gob.mx/gobmx/datosAbiertos.php
Trujillo, O.M.E., O.A.J. Contreras, H.S. Espinosa, P. O. Gutiérrez, T.E. Hernández, N.J. Nava, G.R.G. Martínez, R.R. Martínez, C.J.C. Rojas, H.M. Sánchez, V.R.M. Vigueras, y S.G.Y. Castellanos. 2017. El Verraco. 1ª ed. ED. Universidad Nacional Autónoma de México, Facultad de Medicina Veterinaria y Zootecnia, CU, CDMX.
Villa, N.A., L.E. Sánchez, y A. Ceballos. 2008. Actividad de glutatión peroxidasa y superóxido dismutasa en plasma seminal y sangre en cerdos reproductores. Veterinaria y Zootecnia. 3(1). https://doctoradoagrarias.files.wordpress.com/2015/04/vetzootec-2009.pdf
Vongpralub, T., P. Thananurak, C. Sittikasamkit, P. Chuawongboon, M. Duangjinda, W. Boonkum, and V. Chankitisakul. 2016. Comparison of effects of different antioxidants supplemented to long-term extender on boar semen quality following storage at 17ºC. The Thai Journal of Veterinary Medicine, 46(1):119-126. https://he01.tci-thaijo.org/index.php/tjvm/article/view/49791
Vyt, P., D. Maes, C. Quinten, T. Rijsselaere, W. Deley, M. Aarts, A. De Kruif, and A. Van Soom . 2008. Detailed motility evaluation of boar semen and its predictive value for reproductive performance in sows. Vlaams Dierge Tijds, 77:291-298. https://biblio.ugent.be/publication/746864
Yeste, M., M. Briz, E. Pinart, S. Sancho, E. Bussalleu, and S. Bonet 2010. The osmotic tolerance of boar spermatozoa and its usefulness as sperm quality parameter. Animal Reproduction Science, 119:265-274. https://doi.org/10.1016/j.anireprosci.2010.02.011
Yeste, M., E. Estrada, E. Pinart, S. Bonet, J. Miró, and J. E. Rodríguez-Gil. 2014. The improving effect of reduced glutathione on boar sperm cryotolerance is related with the intrinsic ejaculate freezability. Cryobiology, 68(2):251-261. https://doi.org/10.1016/j.cryobiol.2014.02.004
Yeste M. 2017. State of the art of boar sperm preservation in liquid and frozen state. Animal Reproduction, 14(1):69-81. http://dx.doi.org/10.21451/1984-3143-AR895
Zhang X.G., Q. Liu, L.Q. Wang, G.S. Yang, and J.H. Hu. 2016. Effects of glutathione on sperm quality during liquid storage in boars. Animal Science Journal, 87, 1195-1201. https://doi.org/10.1111/asj.12545
Published
2023-06-15
How to Cite
Abrego-Salgado, Nancy Patricia, Jose Herrera-Camacho, Efren Estrada-Paqui, Juan Carlos Tinoco-Magaña, and Miguel Ángel Bautista Hernandez. 2023. “Effect of Reduced Glutathione (GSH) on the Sperm Quality of Verraco under Subhumid Tropic Conditions”. Archivos Latinoamericanos De Producción Animal 31 (Suplemento), 73-79. https://doi.org/10.53588/alpa.310514.
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Original paper
Copyright (c) 2023 Nancy Patricia Abrego-Salgado, Jose Herrera-Camacho, Efren Estrada-Paqui, Juan Carlos Tinoco-Magaña, Miguel Ángel Bautista Hernandez
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