Determination of gas production in vitro and metabolizable energy content of ruminant feedstuff incubated with sheep excreta
Abstract
An experiment was performed to determine the in vitro gas production at 3, 6, 9, 12, 24, 36 and 48 h of incubation with two types of inoculum collected from two Saint Croix sheep (T1: ruminal liquid, T2: excreta with yeast) in five diets for ruminants with different concentrate (C) and forage (F) ratios (100C:0F, 75C:25F, 50C:50F, 25C:75F y 0C:100F), with the aim to evaluate the feasibility of utilizing excreta instead of ruminal liquid as inoculum in the in vitro gas production (PGIV) technique, in order to determine the in vitro digestibility of organic matter (DIVMO) and metabolizable energy content (EM) in feedstuffs for ruminants. The PGIV registered with ruminal liquid was higher (P < 0.05), than with excreta + yeast as inoculum in each evaluated diet. However, utilizing simple linear regression, polynomial curves or correction factors developed in this research, similar results for PGIV, DIVMO and EM for ruminal liquid were estimated from gas production registered using excreta with yeast as an alternative inoculum.
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Anele, U. Y., K. H. Südekum, J. Hummel, O. M. Arigbede, A. O. Oni, J. A. Olanite, C. Böttger, V. O. Ojo, A. O. Jolaosho. 2011. Chemical characterization, in vitro dry matter and ruminal crude protein degradability and microbial protein synthesis of some cowpea (Vigna unguiculata L. Walp) haulm varieties. Anim. Feed Sci. Technol. 163:161–169.
Arbabi, S., T. Ghoorchi, S. Ramzanpour. 2017. Use of an in vitro rumen gas production technique to evaluate the nutritive value of five forage to concentrate ratios. Iran. J. Appl. Anim. Sci. 7:249–257.
AOAC. 2005. Official Methods of Analysis. 16th ed. Association of Official Analytical Chemists, Arlington, V.A.
Bach, A., C. Iglesias, M. Devant. 2007. Daily rumen pH pattern of loose-housed dairy cattle as affected by feeding pattern and live yeast supplementation. Anim. Feed Sci. Technol. 136:146–153.
Cone, J. W., A. H. Van Gelder, H. Bachmann. 2002. Influence of inoculum source on gas production profiles. Anim. Feed Sci. Technol. 99:221–231.
Dawson, K. A., K. E Newman, J. A. Boling. 1990. Effects of microbial supplements containing yeast and lactobacilli on roughage-fed ruminal microbial activities. Journal of Animal Science. 68: 3392-3398.
El-Meadaway, A., Z. Mir, P. S. Mir, M. S. Zaman, L. J. Yanke. 1998. Relative efficacy of inocula from rumen fluid or faecal solution for determining in vitro digestibility and gas production. Can. J. Anim. Sci. 78:673–679.
Erasmus, L. J., P. M. Botha, A. Kistner. 1992. Effect of yeast culture supplement on production, rumen fermentation, and duodenal nitrogen flow in dairy cows. J. Dairy Sci. 75:3056–3065.
Geron, L. J. V., L. E. C. Veloso, S. C. De Aguiar, A. L. De Souza, I. De Souza Santos, R. J. Trautmann-Machado, A. De Moura Zanine, R. F. Da Silva, D. De Jesus Ferreira, S. F. P. Zanin. 2019. In vitro fermentation of the rations containing Morinda citrifolia L. (Noni) using two types of inoculum. Semin. Agrar. 40:831–842.
Getachew, G., G. M. Crovetto, M. Fondevila, U. Krishnamoorthy, B. Singh, M. Spanghero, H. Steingass, P. H. Robinson, M. M. Kailas. 2002. Laboratory variation of 24 h in vitro gas production and estimated metabolizable energy values of ruminant feeds. Anim. Feed Sci. Technol. 102:169–180.
Hamid, P., T. Akbar, J. Hossein, M. G. Ali. 2007. Nutrient Digestibility and Gas Production of Some Tropical Feeds Used in Ruminant Diets Estimated by the in vivo and in vitro Gas Production Techniques. Am. J. Anim. Vet. Sci. 2:108–113.
Manteca, X. 2012. Capítulo VIII: Bienestar animal. Red Porc. Iberoam. 97–111.
Melesse, A., H. Steingass, M. Schollenberger, M. Rodehutscord. 2017. Screening of common tropical grass and legume forages in Ethiopia for their nutrient composition and methane production profile in vitro. Trop. Grasslands-Forrajes Trop. 5:163–175.
Menke, K.-H., H. Steingass. 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development, 28, 7-55
NOM-062-ZOO-1999, 1999. Norma oficial Mexicana, especificaciones técnicas para la producción, cuidado y uso de animales de laboratorio.
Opsi, F., R. Fortina, S. Tassone, R. Bodas, S. López. 2012. Effects of inactivated and live cells of Saccharomyces cerevisiae on in vitro ruminal fermentation of diets with different forage: concentrate ratio. J. Agric.Sci.150:271–283. https://doi.org/10.1017/S0021859611000578.
Posada, S. L., R. R. Noguera, J. A. Segura. 2012. Ruminant feces used as inoculum for the in vitro gas production technique. Rev. Colomb. Ciencias Pecu. 25:592–602.
Ramin, M., D. Lerose, F. Tagliapietra, P. Huhtanen. 2015. Comparison of rumen fluid inoculum vs. faecal inoculum on predicted methane production using a fully automated in vitro gas production system. Livest. Sci. 181:65–71. https://doi.org/10.1016/j.livsci.2015.09.025.
Storm, I. M. L. D., A. Louise, F. Hellwing, N. I. Nielsen, J. Madsen. 2012. Methods for Measuring and Estimating Methane Emission from Ruminants. Animals. 2:160–183.
Váradyová, Z., M. Baran, I. Zeleňák. 2005. Comparison of two in vitro fermentation gas production methods using both rumen fluid and faecal inoculum from sheep. Anim. Feed Sci. Technol. 123-124 Pa: 81–94.
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