Effect of L-carnitine and melatonin on cryosurvival and fertility of spermatozoa of creole roosters (Gallus gallus domesticus)
Abstract
The production of Creole chicken (Gallus gallus domesticus) in Ecuador is of transcendental importance in the economy and nutrition of rural and city families. Creole chicken has been considered a valuable genetic resource in backyard production, constituting an important economic resource and a basic product in the traditional diet. Therefore, it is necessary to search for biotechnological alternatives to maximize the use of this indigenous genetic material. Sperm cryopreservation and artificial insemination (AI) of birds has been used successfully in hens of different breeds; however, as far as we know, the use of these reproductive biotechnologies in Creole birds in Ecuador is deficient or null. Avian spermatozoa become vulnerable to free radicals (eg H2O2, O2- and OH-) due to polyunsaturated fatty acids (PUFA) present in the plasma membrane. Consequently, ROS easily combine with PUFAs and lead to the production of lipid peroxidation (LPO), causing decreased motility, viability and fertilizing capacity of rooster sperm. In this sense, this research evaluated the effects of ME and LC supplemented to the freezing medium on the cryosurvival and fertility of spermatozoa from Creole roosters (Gallus gallus domesticus).
Downloads
References
Appiah, M. O., He, B., Lu, W., & Wang, J. (2019). Antioxidative effect of melatonin on cryopreserved chicken semen. Cryobiology, 89(December 2018), 90–95. https://doi.org/10.1016/j.cryobiol.2019.05.001
Blesbois, E. (2007). Current status in avian semen cryopreservation. World’s Poultry Science Journal, 63(2), 213–222. https://doi.org/10.1017/S0043933907001419
Chalah, T., Seigneurin, F., Blesbois, E., & Brillard, J. P. (1999). In vitro comparison of fowl sperm viability in ejaculates frozen by three different techniques and relationship with subsequent fertility in vivo. Cryobiology, 39(2), 185-191. https://doi.org/10.1006/cryo.1999.2201
Ehling, C., Taylor, U., Baulain, U., Weigend, S., Henning, M., & Rath, D. (2012). Cryopreservation of semen from genetic resource chicken lines. Agric. For. Res, 62, 151-158.
Fattah, A., Sharafi, M., Masoudi, R., Shahverdi, A., Esmaeili, V., & Najafi, A. (2017). L-Carnitine in rooster semen cryopreservation: Flow cytometric, biochemical and motion findings for frozen-thawed sperm. Cryobiology, 74, 148–153. https://doi.org/10.1016/j.cryobiol.2016.10.009
Lake PE, Ravie O. An exploration of cryoprotective compounds for fowl spermatozoa. Br. Poult Sci. 1984; 25(1):145–150. https://doi.org/10.1080/13632758408454852
Mehaisen, G. M. K., Partyka, A., Ligocka, Z., & Niżański, W. (2020). Cryoprotective effect of melatonin supplementation on post-thawed rooster sperm quality. Animal Reproduction Science, 212. https://doi.org/10.1016/j.anireprosci.2019.106238
Partyka, A., Łukaszewicz, E., and Nizański, W. (2012). Lipid peroxidation and antioxidant enzymes activity in avian semen. Animal Reproduction Science, 134(3–4), 184–190. https://doi.org/10.1016/j.anireprosci.2012.07.007
Surai, P. F. (2015). Antioxidant Action of Carnitine: Molecular Mechanisms and Practical Applications. EC Veterinary Science 2(1): 66-84.
Toalombo, P. A. (2020). Caracterización morfológica, productiva y genética de la gallina criolla del Ecuador. Universidad de Córdoba, UCOPress. https://www.uco.es/ucopress/index.php/es/ucopress@uco.es
Copyright (c) 2022 Diego Andrés Galarza Lucero
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
