Royal jelly supplementation to freezing and vitrification media improves cryosurvival of bull spermatozoa

  • Luis Galarza-Álvarez Facultad de Ciencias Agropecuarias, Universidad de Cuenca
  • Jacqueline Guanga Facultad de Ciencias Agropecuarias, Universidad de Cuenca
  • Juan Lucero Facultad de Ciencias Agropecuarias, Universidad de Cuenca
  • Xavier Samaniego Facultad de Ciencias Agropecuarias, Universidad de Cuenca
  • Diego Andrés Galarza Lucero Universidad de Cuenca
Keywords: cryopreservation, sperm kinematics, Royal jelly, cryosurvival


Royal Jelly (RJ) is a substantial nutrient produced by the hypopharynx and mandibular glands of worker bees from 4 to 11 days of age, and its composition is mainly based on water (60–70%), protein (27–41% ) and carbohydrates (30%), lipids (8–19%), vitamins (A, B, C, E), salts, and amino acids. Due to its composition, JR has been successfully used in sperm cryopreservation at concentrations from 0.2 to 1% (w/v) supplemented with synthetic base extenders (eg TRIS or TALP). Its effect has been evidenced in the increase of motility, viability, functionality of the plasmatic membrane and fertilizing capacity of fresh and cryopreserved ram sperm. Conventional freezing requires the use of penetrating cryoprotectants (eg glycerol) to prevent damage caused by ice crystal formation, however, vitrification requires high concentrations (0.1 to 6 M) of non-penetrating cryoprotectants (eg sucrose or trehalose) and rapid cooling, passing rapidly to the glass transition without forming ice crystals. This research evaluated the effect of JR (0.2%) supplemented to freezing (TCG-YH + 5% glycerol) and vitrification (TCG-YH + 100 mM sucrose) media on cryosurvival of bull sperm.


Download data is not yet available.

Author Biography

Diego Andrés Galarza Lucero, Universidad de Cuenca

Profesor Titular Agregado 1, Facultad de Cienicas Agropecuarias, Escuela de Medicina Veterinaria y Zootecnia


Abd-Allah, S. M. (2010). Effect of royal jelly on bovine sperm characteristics during post-thaw incubation in vitro. Rev Vet, 21(2), 81-5.
Abd-Allah, S. M. (2012). Effect of royal jelly on the fertilizing ability of buffalo spermatozoa in vitro. Journal of Buffalo Science, 1(1), 1-4.
Amini, S., Masoumi, R., Rostami, B., Shahir, M. H., Taghilou, P., & Arslan, H. O. (2019). Effects of supplementation of Tris-egg yolk extender with royal jelly on chilled and frozen-thawed ram semen characteristics. Cryobiology, 88, 75-80.
El-Sherbiny, A. (2013). Effect of royal jelly in honey-included egg yolk based extenders on motility, viability and fertilizing ability of frozen rabbit spermatozoa. Egyptian Journal of Rabbit Science, 23(2), 149-160. DOI: 10.21608/ejrs.2013.54261
Guanga, J., Lucero, J., Samaniego, J.X., Segarra, E., Galarza-Álvarez, L., Galarza, D.A. (2022). 2210. La suplementación de jalea real a diluyente de base sintética y no sintética ayuda a mantener la motilidad y cinética de esperma de toro refrigerado. Proceedings del Primer Simposio Internacional de Reproducción Animal en Ecuador (IRAE). SPERMOVA, 12(1), 170-171.
Holt, W. V., Penfold, L. M. (2014). Animal Andrology: Theories and applications, In: S. Chenoweth (Ed.), Anim. Androl. Theor. Appl, 2014, pp. 78–81. Boston, MA, USA.
Maghsoudlou, A., Mahoonak, A. S., Mohebodini, H., & Toldra, F. (2019). Royal jelly: chemistry, storage and bioactivities. Journal of apicultural Science, 63(1), 17-40.
Moradi, A. R., Malekinejad, H., Farrokhi-Ardabili, F., & Bernousi, I. (2013). Royal Jelly improves the sperm parameters of ram semen during liquid storage and serves as an antioxidant source. Small ruminant research, 113(2-3), 346-352.
Moutsatsou, P., Papoutsi, Z., Kassi, E., Heldring, N., Zhaohao, C., Tsiapara, A., ... Dahlman-Wright, K. (2010). Fatty acids derived from royal jelly are modulators of estrogen receptor functions. Plos One, 5, 15594.
Sakhdary, H., Farshad, A., Rostamzadeh, J., Binabaj, F. B., & Sobhani, K. (2022). Effects of enzymatic and non-enzymatic antioxidants in diluents on cryopreserved bull epididymal sperm. Asian Pacific Journal of Reproduction, 11(1), 44. DOI: 10.4103/2305-0500.335861
How to Cite
Galarza-Álvarez, Luis, Jacqueline Guanga, Juan Lucero, Xavier Samaniego, and Diego Andrés Galarza Lucero. 2022. “Royal Jelly Supplementation to Freezing and Vitrification Media Improves Cryosurvival of Bull Spermatozoa ”. Latin American Archives of Animal Production 30 (Supl. 2), 87-89.