Royal jelly supplementation to freezing and vitrification media improves cryosurvival of bull spermatozoa

  • Luis Galarza-Álvarez Facultad de Ciencias Agropecuarias, Universidad de Cuenca
  • Jacqueline Guanga Facultad de Ciencias Agropecuarias, Universidad de Cuenca
  • Juan Lucero Facultad de Ciencias Agropecuarias, Universidad de Cuenca
  • Xavier Samaniego Facultad de Ciencias Agropecuarias, Universidad de Cuenca
  • Diego Andrés Galarza Lucero Universidad de Cuenca
Keywords: cryopreservation, sperm kinematics, Royal jelly, cryosurvival


Royal Jelly (RJ) is a substantial nutrient produced by the hypopharynx and mandibular glands of worker bees from 4 to 11 days of age, and its composition is mainly based on water (60–70%), protein (27–41% ) and carbohydrates (30%), lipids (8–19%), vitamins (A, B, C, E), salts, and amino acids. Due to its composition, JR has been successfully used in sperm cryopreservation at concentrations from 0.2 to 1% (w/v) supplemented with synthetic base extenders (eg TRIS or TALP). Its effect has been evidenced in the increase of motility, viability, functionality of the plasmatic membrane and fertilizing capacity of fresh and cryopreserved ram sperm. Conventional freezing requires the use of penetrating cryoprotectants (eg glycerol) to prevent damage caused by ice crystal formation, however, vitrification requires high concentrations (0.1 to 6 M) of non-penetrating cryoprotectants (eg sucrose or trehalose) and rapid cooling, passing rapidly to the glass transition without forming ice crystals. This research evaluated the effect of JR (0.2%) supplemented to freezing (TCG-YH + 5% glycerol) and vitrification (TCG-YH + 100 mM sucrose) media on cryosurvival of bull sperm.


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Author Biography

Diego Andrés Galarza Lucero, Universidad de Cuenca

Profesor Titular Agregado 1, Facultad de Cienicas Agropecuarias, Escuela de Medicina Veterinaria y Zootecnia


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How to Cite
Galarza-Álvarez, Luis, Jacqueline Guanga, Juan Lucero, Xavier Samaniego, and Diego Andrés Galarza Lucero. 2022. “Royal Jelly Supplementation to Freezing and Vitrification Media Improves Cryosurvival of Bull Spermatozoa ”. Archivos Latinoamericanos De Producción Animal 30 (Supl. 2), 87-89.