Productive behavior and nutritional quality of two varieties of alfalfa (Medicago sativa L.) subjected to fertilization and inoculation

  • Guido Arnaldo Portillo Facultad de Ciencias Agrarias, Universidad Nacional de Asunción https://orcid.org/0000-0003-0749-0826
  • Pedro Luis Paniagua Alcaráz Facultad de Ciencias Agrarias, Universidad Nacional de Asunción
Keywords: Medicago sativa, Meagronomic performance, nutritional quality, fertilizers, Bradyrhizobium japonicum

Abstract

The objective of the research was to evaluate the productive performance and nutritional quality of two varieties of alfalfa subjected to fertilization and inoculation. The experiment consisted of two phases (growth and regrowth) and the experimental design was completely randomized, with a 2 x 2 x 2 factorial arrangement. The factors were: varieties (Pampeana9 - P9) and Roundup Ready - RR), with or without fertilization and with or without inoculation, resulting in eight combinations and four repetitions. Plant height (AP), coverage (%), dry matter percentage (%DM), green and dry mass yield (RMV and RMS), crude protein content (CP), total digestible nutrients (NDT) were measured. ), metabolizable energy (ME), non-fibrous carbohydrates (CNF), ether extract (EE), mineral matter (MM), neutral and acid detergent fiber (NDF and FDA), lignin (Lig) and acid detergent insoluble protein (PIDA ). The P9 variety performed better for the MM and PIDA variables, presenting lower values (11.60 and 11.40% respectively). Fertilization improved the CP (0.25 percentage point), the AP up to 75 DDE (12%), the coverage in both phases (9 and 4 percentage points), the RMV (27%) and the RMS (36%) in the growth phase, but reduced the %DM in this phase (3 percentage points). The inoculation reduced the coverage in both phases (7 and 4 percentage points), the EE, FDN, FDA, Lig and PIDA by 0.25; 1.84; 3.22; 2.28 and 1.46 percentage points, respectively; A lower RMV (30%) was also observed in the growth phase; but it improved the %DM (1 percentage point), RMV (20%) and RMS (23%) in the regrowth phase and increased the MM content (0.78 percentage point).

Downloads

Download data is not yet available.

References

Aguilar-Quintana, ED. 2017. producción de biomasa forrajera de variedades o ecotipos de alfalfa (Medicago Sativa L.) en el sector humedades del distrito de Salas – Lambayeque. Tesis (Ingeniero Zootecnista). Universidad Nacional “Pedro Ruiz Gallo”. Facultad de Ingeniería Zootecnia. PE, Lambayeque: FCZ – UNPRG, 72 págs. https://repositorio.unprg.edu.pe/bitstream/handle/20.500.12893/1866/BC-TES-TMP-699.pdf?sequence=1&isAllowed=y

Alberto, A, JC Cruz, AC Pantoja, RM Lopez, W Bermúdez y E Morales. 2021. Estudio comparativo de la producción de forraje y calidad nutricional de variedades de cultivo de alfalfa (Medicago sativa), en la puna húmeda y seca del Perú. Compendio Cienc. Veterinario, 11(2):7-12. https://doi.org/10.18004/compend.cienc.vet.2021.11.02.7

Association Analytical Chemists (AOAC). 2007. Official methods of analysis 18.ed. Gaithersburg, Maryland, USA; AOAC international, págs. 21 - 22. ISBN-10:‎ 0935584757.

Bai, XJ, BR Wang, SS An, QC Zeng y HX Zhang. 2019. Response of forest species to C:N:P in the plant–litter–soil system and stoichiometric homeostasis of plant tissue during afforestation on the Loess Plateau, China. CATENA 183: 104186. https://doi.org/10.1016/j.catena.2019.104186

Brady, NC y RR Weil. 2013. Elementos da natureza e propiedades dos solos. 3. ed. Porto Alegre: Bookman, 790 págs. ISBN: 978-85-65837-74-3.

Cabral, WB, AL Souza, E Alexandrino, FB Toral, J Santos y MV Carvalho. 2012. Características estruturais e agronômicas da Brachiaria brizantha cv. Xaraés submetida a doses de nitrogênio. Revista Brasileira de Zootecnia, 41(4):846-855. https://doi.org/10.1590/S1516-35982012000400004

Centeno, LN; Guevara, MDF; Cecconello, ST; Sousa, ROD; Timm, LC. 2017. Textura do solo: Conceitos e Aplicações em Solos Arenosos. Revista Brasileira de Engenharia e Sustentabilidade, 4(1):31-37. https://periodicos.ufpel.edu.br/ojs2/index.php/RBES/article/view/11576

Cherrad, S, A Charnay, C Hernandez, H Steva, L Belbahri y S Vacher. 2018. Emergence of boscalid-resistant strains of Erysiphe necator in French vineyards. Microbiol Res, 216:79–84. https://doi.org/10.1016/j.micres.2018.08.007

Comeron, EA, R Ferreira, D Vilela, FA Kuwahara y O Tupy. 2015. Utilização da alfafa em pastejo para alimentação de vacas leiteiras. In: Ferreira, D Vilela, EA Cameron, AC Bernardi y D Karam, D. (Ed.). Cultivo e utilização da alfafa em pastejo para alimentação de vacas leiteiras. Brasília, DF: Embrapa Sede, 131-149 págs.

Corsi, M; Martha JR., GB; Pagotto, DS. 2001. Sistema radicular: dinâmica e resposta a regimes de desfolha. In: Mattos, WRS. (Ed.) A produção animal na visão dos brasileiros Piracicaba: Fundação de Estudos Agrários Luiz de Queiroz, 838-852 págs.

Dalla-Fontana, LA, I Nescier, DT Pennisi, ML Longoni y L Contini. 2007. Efecto de la fertilización sobre la calidad de plantas de alfalfa (Medicago sativa L.) a través del ciclo de producción. Revista FAVE - Ciencias Veterinarias 6(1-2): 87-93. https://doi.org/10.14409/favecv.v6i1/2.1445

Detmann, E, MA Souza, SC Valadares-Filho, AC Queiroz, TT Berchielli, EOS Saliba, LS Cabral, DS Pina, MM Ladeira y JAG Azevedo. 2012. Métodos para análise de alimentos. Suprema: Visconde do Rio Branco, 214 págs. ISBN: 9788581790206.

Di Rienzo, JA, F Casanoves, MG Balzarini, L Gonzalez, M Tablada y CW Robledo. 2013. InfoStat versión 2013. Grupo InfoStat, FCA. Universidad Nacional de Córdoba, Argentina. Disponible en: http://infostat.com.ar

Dias, P, S Camargo-Trabali, S Aronovich, M Aronovich, S Souto y L Schimidt. 2002. Avaliação de vinte oito cultivares de alfafa em paty do alferes, Rio de Janeiro. Agronomia, 36(1/2):29-36. http://www.ia.ufrrj.br/revista/artigos/2002-12/20_27.pdf

Duarte, E. 2020. Manejo da adubação na cultura de alfafa. Dissertação (Mestrado em Agronomia). Universidade Estadual do Norte do Paraná, Campus Luiz Meneghel, 105 págs. https://uenp.edu.br/dissertacao-agronomia/16386-erich-dos-reis-duarte/file

Flórez-Delgado, DF. 2015. La alfalfa (Medicago sativa): origen, manejo y producción. Conexagro JDC, 5(1):27–43. https://1library.co/document/zp0m7moq-la-alfalfa-medicago-sativa-origen-manejo-y-produccion.html

Gallego, JJ. 2017. Efectos de la fertilización con P y S sobre la producción y calidad de alfalfa (Medicago sativa L.) irrigada y el estado orgánico del suelo en el valle inferior del Río Negro. Tesis (Magister en Ciencias Agrarias). Universidad Nacional del Sur. AR, Bahía Blanca: UNS, 87 págs. https://repositoriodigital.uns.edu.ar/bitstream/handle/123456789/3593/Tesis%20final%20%28imprimir%2004-7-2017%29.pdf?sequence=1&isAllowed=y

Gaviria, X, J Rivera y R Barahona. 2015. Calidad nutricional y fraccionamiento de carbohidratos y proteína en los componentes forrajeros de un sistema silvopastoril intensivo. Pastos y Forrajes, 38(2):194-201. http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0864-03942015000200007

Glienke, CL, AB Soares, V Biezus, TS Assmann, AL Assmann y TD Semler. 2013. Teores de potássio no solo cultivado com alfafa em função de doses de adubação potássica em sistema de plantio direto. In: XXXIV Congresso Brasileiro de Ciencia do Solo, 1-4 págs. https://www.eventossolos.org.br/cbcs2013/anais/arquivos/2635.pdf

Grewal, HS. 2001. Alfalfa responses to combined use of lime and limiting nutrients on an acidic soil. Communications in Soil Science and Plant Analysis, 41(19):2247-2263. https://www.tandfonline.com/doi/abs/10.1080/00103624.2010.507832

Guevara-Mesa, AL, LA Miranda-Romero, JE Ramírez-Bribiesca, SS González-Muñoz, MM Crosby-Galvan, LM Hernández-Calva y OE Del Razo-Rodríguez. 2011. Protein fractions and in vitro fermentation of protein feeds for ruminants. Tropical and Subtropical Agroecosystems, 14 (2011): 421-429. https://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1870-04622011000200008

Jafari, M, M Yari, M Ghabooli, M Sepehric, E Ghasemi y A Jonke. 2018. Inoculation and co-inoculation of alfalfa seedlings with root growth promoting microorganisms (Piriformospora indica, Glomus intraradices and Sinorhizobium meliloti) affect molecular structures, nutrient profiles and availability of hay for ruminants. Animal Nutrition, 4(1): 90-99. https://doi.org/10.1016/j.aninu.2017.08.008

Licitra, G, TM Hernandez y PJ Van Soest. 1996. Standardizations of procedures for nitrogen fractionation of ruminant feeds. Animal Feed Science and Technology, 57:347-358. https://doi.org/10.1016/0377-8401(95)00837-3

Liu, J, L Tang, H Gao, M Zhang y C Guo. 2018. Enhancement of alfalfa yield and quality by plant growth-promoting rhizobacteria under saline-alkali conditions. Journal of the Science of Food and Agriculture, 33(1):281-289. https://doi.org/10.1002/jsfa.9185

López, O, E González, P Llamas, A Molinas, E Franco, S García y E Ríos. 1995. Estudio de reconocimiento de suelos, capacidad de uso de la tierra y propuesta de ordenamiento territorial preliminar de la Región Oriental del Paraguay. Asunción, PY, MAG/Banco Mundial. 284 págs.

Martínez, BÁ y MM Leiva. 2018. Estudio comparativo de la producción de forraje y calidad nutricional de variedades de cultivo de alfalfa (Medicago sativa), en la Sierra Central. Tesis (Ingeniero Agrónomo). Universidad Nacional Daniel Alcides Carrión. Facultad de Ciencias Agropecuarias. PE, Pasco: UNDAC – FCA, 77 págs. https://1library.co/document/y4glo30y-estudio-comparativo-produccion-forraje-calidad-nutricional-variedades-medicago.html

National Research Council (NRC). 1989. Nutrient Requirements of Dairy Cattle. (6th Ed.). National Academy Press, Washington, DC, 157 págs.

Ormeño-Orrillo, E, M Hungria y E Martínez-Romero. 2013. Dinitrogen-fixing prokaryotes. In: Rosemberg, E, EF De Long, S Lory, E Stackebrandt, y F Thompson (Ed.). 2013. The Prokayotes: prokaryotic physiology and biochemistry. Berlin Heidelberg: Springer-Verlag, 427-451 págs.

Rebelato, RC. 2014. Efeito da nova técnica de coinoculaçào na cultura da alfafa (Medicago sativa), com Rhizobium e Azospirillum. Projeto de Trabalho de Conclusão de Curso (Bacharel em Ciências Rurais). Universidade Federal de Santa Catarina, UFSC, 15 págs. https://repositorio.ufsc.br/bitstream/handle/123456789/127547/Renan_Carlos_Rebelato.pdf?sequence=1&isAllowed=y

Ribeiro, A. 2021. Brachiaria brizantha cv. Marandu sob diferentes adubações e intensidades de corte. Dissertação (Mestre em Zootecnia). Universidade Estadual do Sudoeste da Bahia, 60 págs. http://www2.uesb.br/ppg/ppz/wp-content/uploads/2021/06/Disserta%C3%A7%C3%A3o-Amanda-Corrigida-Final-PPZ.pdf

Rojas-García, AR, A Hernández-Garay, SJ Cansino, M Maldonado-Peralta, SI Mendoza-Pedroza, P Álvarez-Vázquez y BM Joaquín-Torres. 2016. Comportamiento productivo de cinco variedades de alfalfa. Revista Mexicana de Ciencias Agrícolas, 7(8), 1855-1866. http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S2007-09342016000801855&lng=es&tlng=es

Rojas-García, AR, N Torres-Salado, S Joaquín-Cancino, A Hernández-Garay, M Maldonado-Peralta y P Sánchez-Santillán. 2017. Componentes del rendimiento en variedades de alfalfa (Medicago sativa L.). Agrociencia, 51: 697-708. http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952017000700697

Samira, S, H Cherif-Silini, A Chenari-Bouket, A Silini, M Eshelli, L Luptakova, F Alenezi y L Belbahri. 2021. Improvement of Medicago sativa Crops Productivity by the Co-inoculation of Sinorhizobium meliloti–Actinobacteria Under Salt Stress. Current Microbiology, 78:1344–1357. https://doi.org/10.1007/s00284-021-02394-z

Salton, JC y M Tomazi. 2014. Sistema Radicular de Plantas e Qualidade do Solo. Dourados, MS: EMBRAPA: Comunicado técnico Nº 198, 7 págs. ISSN: 1679-0472.

Sánchez-Santillán, P, M Maldonado-Peralta, AR Rojas-García, NTorres-Salado, J Herrera-Pérez, MB Bottini-Luzardo, CY Wilson-García y AR Quero-Carrillo. 2019. Productividad de variedades de alfalfa en el Valle de México. Acta Universitaria, 29, e2202. http://doi.org/10.15174.au.2019.2202

Santos, IG. 2017. Seleção de caracteres complexos em alfafa por meio de inteligência computacional. Dissertação (Mestrado em Genética e Melhoramento). Universidade Federal de Viçosa. Viçosa, MG: UFV, 91 págs. https://www.locus.ufv.br/bitstream/123456789/16369/1/texto%20completo.pdf

Silva, LA. 2020. Produção de biomassa e composição bromatológica da alfafa submetida a coinoculação com bacterias. Dissertacao (Mestrado). Universidade Estadual Paulista (UNESP). Faculdade de Ciências Agrárias e Tecnológicas de Dracena. BR, Sao Paulo: UNESP, 52 págs. https://repositorio.unesp.br/handle/11449/193018

Stavarache, M, J Muntianu, V Vîntu, C Samuil y CI Popovici. 2010. Research on the influence of inoculation and fertilization on the morphological and productive features of lucerne (Medicago sativa L.) under conditions of moldavian forest steppe. Lucrări Ştiinţifice, 53(2): 243 – 248. http://www.uaiasi.ro/revagrois/PDF/2010_2_245.pdf

Sulca, A. 2015. “producción en forraje de cinco variedades de alfalfa (Medicago sativa L). Ticllas a 2395 msnm - Ayacucho”. Tesis (Ingeniero Agrónomo). Universidad Nacional de San Cristobal de Huamanga. Facultad de Ciencias Agrarias. PE; Ayacucho: UNSC – FCA, 121 págs. http://repositorio.unsch.edu.pe/bitstream/UNSCH/3122/1/TESIS%20AG1198_Sul.pdf

Thang, PT, NV Vien y TD Khanh. 2021. “Allelopathic potential of an invasive plant (Cassytha Filiformis L.) under different assessing conditions”. Plant Cell Biotechnology and Molecular Biology, 22: 17-18. https://www.ikppress.org/index.php/PCBMB/article/view/6075

Tupy, O, R Ferreira, D Vilela y FA Kuwaraha. 2015. Viabilidade econômica e financeira do pastejo em alfafa em sistemas de produção de leite. EMBRAPA Pecuária Sudeste. Comunicado técnico Nº 118, 57 págs. https://ainfo.cnptia.embrapa.br/digital/bitstream/item/144730/1/Documentos118-Ainfo.pdf

Van Soest, PJ. 1994. Nutritional ecology of the ruminant. 2º ed, Cornell University Press, Ithaca, NY. pp 436. eISBN: 978-1-5017-3235-5.

Van Soest, PJ, JB Robertson y BA Lewis. 1991. Methods for dietary fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science. 74: 3583-3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2

Weiss, WP. 1993. Predicting energy value of feeds. Journal of Dairy Science, 76:1802-1811. https://doi.org/10.3168/jds.S0022-0302(93)77512-8

Zhou, J, D Bo, Y Zhang, A Cobb y Z Zhang. 2017. Molybdate in Rhizobial Seed-Coat Formulations Improves the Production and Nodulation of Alfalfa. Plos One, 12 (1): e0170179. https://doi.org/10.1371/journal.pone.0170179

Published
2024-09-13
How to Cite
Portillo, Guido Arnaldo, and Pedro Luis Paniagua Alcaráz. 2024. “Productive Behavior and Nutritional Quality of Two Varieties of Alfalfa (Medicago Sativa L.) Subjected to Fertilization and Inoculation”. Archivos Latinoamericanos De Producción Animal 32 (3 in progr), 145-54. https://doi.org/10.53588/alpa.320303.
Section
Original paper