FR 31. ELISA vs RIA techniques comparation in the plasmatic progesterone determination in Bovines

Abstract

Plasma progesterone (P4) concentrations using ELISA kit and RIA kit were examined in 56 samples of bovine heifers. The intra- and inter-assay coefficients of variation were 5.77 %, 6.50 % and 5.85, 8.50 % respectively, based on two plasma samples. There was a significant correlation between ELISA and RIA results based on 56 plasma samples (r=0.933; P<.05). Simple linear regression, two samples t test and one way analysis of variance indicated that there were not significant differences between ELISA and RIA. Serum P4 concentrations examined in non-pregnant heifers were normal changes of P4 levels observed during all the estrous cycles. Comparison of The ELISA system with the RIA system gave a regression linear ELISA = 2.7001 + 1.2431X (r2=0.89). Total time needed for the ELISA assay was approximately 1½ h. It was concluded that ELISA kit was available for measuring P4 concentrations in bovine heifers. In general, ELISA also offers the advantages of less expensive detector instrumentation, longer shelf-life of reagents and more simplified assay protocols, which can potentially lead to greater automation, than RIA.