Response of tall fescue free and infected with endophyte in pure pastures and in mixture with legumes under two frequencies of defoliation

  • Lucas Ricardo Petigrosso Facultad de Ciencias Agrarias - Universidad Nacional de Mar del Plata
  • María Irastorza Universidad Nacional de Mar del Plata
  • Osvaldo Ramón Vignolio Universidad Nacional de Mar del Plata
  • Silvina San Martino Universidad Nacional de Mar del Plata
  • Mabel Noemí Colabelli Universidad Nacional de Mar del Plata
  • Silvia Graciela Assuero Universidad Nacional de Mar del Plata
Keywords: Festuca, Epichloë coenophiala, Lotus tenuis, Trifolium repens, dilution effect


The objectives of the present work were to compare the effect of two defoliation frequencies on the aerial biomass accumulation of a) pure stands of two types of Schedonorus arundinaceus (tall fescue), one from seed harvested from randomly selected individual plants of a natural pasture infected with wild endophyte Epichloë coenophiala (FNE+) and another from a commercial endophyte-free cultivar (FCE-) (Experiment 1) and b) mixed stands of FCE- and FNE+ with legumes (Lotus tenuis and Trifolium repens) (Experiment 2); and c) tall fescue root biomass production in pure and mixed stands above. Two field experiments were carried out. The experiments were conducted in a completely randomized design with a factorial arrangement of two factors: type of tall fescue (FNE+ and FCE-) and frequency of defoliation (AF: high; BF: low), with three replicates. Five and 9 cuts were carried out in BF and AF treatments, respectively (cutting height 70 mm from the ground level). Immediately after the last aerial biomass harvest, tall fescue root biomass was determined in both experiments. Along the experimental period total accumulated aerial biomass production of tall fescue stands (both in pure and mixed stands) was not affected (p>0.05) by the frequency of defoliation nor the type of tall fescue. No significant effects (p>0.05) of the frequency of defoliation and type of tall fescue were detected on root biomass of tall fescue, neither in pure nor in mixed stands.


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Author Biography

Lucas Ricardo Petigrosso, Facultad de Ciencias Agrarias - Universidad Nacional de Mar del Plata

Lucas Ricardo Petigrosso is currently an assistant Professor of Ecology in Faculty of Agrarian Science of the National University of Mar del Plata (FCA-UNMdP), Argentina. He received a B.S. degree in Agricultural (2011) and MSc in Crop Production (2015) from FCA-UNMdP. He has done research on how the various ecological factors influence in the process of transmission of the asymptomatic endophytic fungi in grasses such as tall fescue. This information is indispensable to facilitate the management of symbiosis in productive contexts. He was Associate Editor of Phyton (International Journal of Experimental Botany). He currently is also an editor board member of Journal of Biotechnology Research, Annals of Ecology and Environmental Science and Reviewer of various Journals. He is an evaluator in Science and Technology Fair; major Professor B.S. Thesis in FCA-UNMdP; author and Co-author of publications in refereed scientific journals, Proceedings of National and International Congress.


Agnusdei M.G. and A. Mazzanti. 2001. Frequency of defoliation of native and naturalized species of the Flooding Pampas (Argentina). Grass and Forage Science 56: 344-351.

Allen, R.G., L.S. Pereira, D. Raes and M. Smith. 1998. Crop evapotranspiration-Guidelines for computing crop water requirements-FAO Irrigation and drainage paper 56. FAO, Roma, vol. 300, no 9, p. D05109.

Assuero, S.G., J.A. Tognetti, M.R. Colabelli, M. G. Agnusdei, E. C. Petroni and M. A. Posse. 2006. Endophyte infection accelerates morpho-physiological responses to water deficit in tall fescue. New Zealand Journal of Agricultural Research 49: 359-370.

Bell, C.C. 1985. Effect of defoliation frequency on simulated swards of ryegrass, tall fescue, and a 50/50 mixture of the two species. New Zealand Journal of Agricultural Research 28: 307-312.

Borrajo, C. I. 2015. Implantación de cultivares de festuca en la Cuenca del Salado. Recuperado de

Clay, K. and C. Schardl. 2002. Evolutionary origins and ecological consequences of endophyte symbiosis with grasses. American Naturalist 160: 99-127.

Dawson, L.A., S.J. Grayston and E. Paterson. 2000. Effects of grazing on the roots and rhizosphere of grasses CAB International 2000. En: Lemaire, G. et al. (Eds.) Grassland ecophysiology and grazing ecology (pp. 61-84). CABI Publishing, New York.

Elizalde, J. y Riffel, S. 2015. Alertan sobre festucosis en la Cuenca del Salado. [en línea] [consulta: 20 de febrero de 2015].

Evans, T.J., G.E. Rottinghaus and S.W. Casteel. 2004. Ergot. En: Plumlee, K.H. (Ed.) Clinical Veterinary Toxicology (pp. 239–243). Mosby, St. Louis, MO.

Evans, T.J., D.J. Blodgett and G. E. Rottinghaus. 2012. Fescue toxicosis. En: Gupta, R.C. (Ed.) Veterinary toxicology. (pp. 1166-1180). Academic Press, San Diego, CA.

Ferraro, D.O. and M. Oesterheld. 2002. Effect of defoliation on grass growth. A quantitative review. Oikos 98: 125-133.

Formoso, F. 2010. Festuca arundinacea, manejo para producción de forraje y semillas. Programa Nacional de Pasturas y Forrajes. INIA La Estanzuela. 200 p.

García, J.A., G. Piñeiro, S. Arana and F. H. Santiñaque. 2010. Moisture Deficit and Defoliation Effects on White Clover Yield and Demography. Crop Ecology, Management & Quality 50 (5): 2009-2020.

García, J.A., J.C. Canton, B.L. Garcïa, J.F. Micheloud, C.M. Campero, E.J.A. Spath and E.R. Odrizola. 2017. Retrospective analysis of cattle poisoning in Argentina (2000-2013). Revista Pesquisa Veterinária Brasileira 37: 210-214.

Gittins, C.; C.A. Busso, G. Becker, L. Ghermandi, G. Siffredi, G. 2010. Defoliation frequency affects morphophysiological traits in the bunchgrass Poa ligularis. International Journal of Experimental Botany 79: 55-68.

Glenn, A.E., C.W. Bacon, R. Price and R.T. Handil. 1996. Molecular phylogeny of Acremonium and its taxonomic implications. Mycologia 88: 369-383.

Hill, N.S., W.C. Stringer, G.E. Rottinghaus, D.P. Belesky, W.A. Partot and D.D. Pope. 1990. Growth, morphological and chemical component responses of tall fescue to Acremonium coenophialum. Crop Science 30: 156-161.

Hoveland, C.S., J.H. Bouton and R.G. Durham. 1999. Fungal endophyte effects on production of legumes in association with tall fescue. Agronomy Journal 91: 897-903.

Kerrisk, J.J. and N.A. Thomson. 1990. Effect of intensity and frequency of defoliation on growth of ryegrass, tall fescue and phalaris. Proceedings New Zealand Grasslands Association 51: 135-138.

Leuchtmann, A, C.W. Bacon, C.L. Schardl, J.F. Jr. White and M. Tadych. 2014. Nomenclatural realignment of Neotyphodium species with genus Epichloë. Mycology 106: 202–215.

Malinowski, D. and D. Belesky. 2000. Adaptations of endophyte infected cool-season grasses to environmental stresses: mechanisms of drought and mineral stress tolerance. Crop Science 40: 923-940.

Manzini, N.A. 1991. Estudios sobre Acremonium sp. en festuca. INIA. Unidad de Difusión e Información Tecnológica. Montevideo-Uruguay. Serie Técnica Nº 8. 16 p.

Petigrosso, L.R., M.N. Colabelli, O.N. Fernández, V. Ispizúa and M.G. Cendoya. 2013. Incidence of the endophyte fungus Neotyphodium coenophialum in pastures of tall fescue differing in age and soil characteristics. African Journal of Agricultural Research 8: 2655-2662.

Petigrosso, L.R., S.G. Assuero, O.R. Vignolio, Y. Romano, M.N. Colabelli, A. Salvat and D. Cristos. 2018. Interacción entre festuca alta infectada con endófito y Lotus tenuis bajo dos frecuencias de defoliación. Revista de Investigaciones Agropecuarias 44(1): 41-48.

R. Development Core Team. 2018. R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna.

Saha, C.D., M.A. Jackson and J.M. Johnson-Cicalese. 1988. A rapid staining method for detection of endophytic fungi in turf and forage grass. Phytopathology 78: 237-239.

Saikkonen, K., P. Wali, M. Helander and S.H. Faeth. 2004. Evolution of endophyte-plant symbioses. Trends Plant Science 9: 275–280.

Salminen, S.O. and P.S. Grewal 2002. Does decreased mowing frequency enhance alkaloid production in endophytic tall fescue and perennial ryegrass? Journal of Chemical Ecology 28: 939–950.

Salminen, S.O., P.S. Grewal and M. F. Quigley. 2003. Does mowing height influence alkaloid production in endophytic tall fescue and perennial ryegrass? Journal of Chemical Ecology 29: 6-10.

Schardl, C.L., C.A. Young, J. Pan, S. Florea, J.E. Takach, D.G. Panaccione, M. Farman, J. Webb, J. Jaromczyk, N. Charlton, P. Nagabhyru, L. Chen, C. Shi and A. Leuchtmann. 2013. Currencies of mutualisms: sources of alkaloid genes in vertically transmitted Epichloe. Toxins 5: 1064-1088. .

Scheneiter, J.O., I.I. Kaufmann, A.R. Ferreyra and R.T. Llorente. 2015. The herbage productivity of tall fescue in the Pampas region of Argentina is correlated to its ecological niche. Grass Forage Science 71: 403-412.

Schiller, J.M.A. and A. Lazenby. 1975. Yield performance of tall fescue (Festuca arundinacea Schreb.) population on the Northern Tablelands of New South Wales. Australian Journal of Experimental Agriculture 15: 391-397.

Shelby, R.A. and L.W. Dalrymple. 1987. Incidence and distribution of the tall fescue endophyte in the United States. Plant Disease 71: 783-786.

Siegel, M.R., G.C.M. Latch and M. C. Johnson. 1987. Fungal endophytes of grasses. Annual Review of Phytopathology 25: 193-315.

Stuedemann, J.A. and D.H. Seman. 2005. Integrating genetics, environment, and management to minimize animal toxicoses. En: Roberts, C.A., West, C.P., Spiers, D.E. (Eds). Neotyphodium in Cool-Season Grasses. (pp. 305-324). Blackwell, Oxford, UK.

Turner, L.R., M.M. Holloway-Phillips, R.P. Rawnsley, D.J. Donaghy and K.G. Pembleton. 2012. The morphological and physiological responses of perennial ryegrass (Lolium perenne L.), cocksfoot (Dactylis glomerata L.) and tall fescue (Festuca arundinacea Schreb, syn. Schedonorus phoenix Scop.) to variable water availability. Grass Forage Science 67: 507-518.

Vignolio, O.R., L. Petigrosso, I. Rodríguez and N. Murillo. 2017. Effects of temperature, photoperiod and defoliation on flowering time of Lotus tenuis (Fabaceae) in Buenos Aires, Argentina. Experimental Agriculture 54: 417-427.

White, J.F. Jr. and M.S. Torres. 2009. Defensive mutualism in microbial symbiosis. CRC Press. Boca Raton, FL. 430 p.

How to Cite
Petigrosso, Lucas Ricardo, María Irastorza, Osvaldo Ramón Vignolio, Silvina San Martino, Mabel Noemí Colabelli, and Silvia Graciela Assuero. 2021. “Response of Tall Fescue Free and Infected With Endophyte in Pure Pastures and in Mixture With Legumes under Two Frequencies of Defoliation”. Latin American Archives of Animal Production 30 (1), 19-28.
Original paper