Differentiation and genetic distance between Venezuelan Creole pigs
Abstract
In order to analyze the differentiation and genetics distance between Venezuelan Creole pigs, hairless samples were taken from individuals from Capanaparo (n = 29), Cunaviche (n = 32), Guadarrama (n = 31), El Socorro (n = 26), Hato Masaguaral (n = 22), Guayabal (n = 31), as well as Landrace (n = 21), Large White (n = 14), Alentejano (n = 29) and Ibérico (n = 30) reference groups. In the Laboratory of Molecular Genetics, Center for Research in Agricultural Biotechnology, Institute of Genetics Faculty of Agronomy, Central University of Venezuela, DNA was extracted from the hair follicles. Were used 13 microsatellite markers: S0155, S0215, S0218, S0225, S0227, SW24, SW240, SW632, SW857, SW911, SW936 y SW951. The fragments were amplified by PCR technique, vertical electrophoresis was performed on polyacrylamide gels, and the fragments was revealed with silver nitrate. The “F”, “GST” statistics was calculated and the multiple correspondence factorial analyses was performed, the DC and DA distances were obtained and the dendrogram was constructed using neighbor joining methodology. The “FIT” ranged between 0.029 and 0.414; “FIS” between -0.126 and 0.414; “FST” between 0.026 and 0.241; “GST” between 0.043 and 0.0293. The smallest distances was observed between El Socorro and Arismendi pigs, and these turn close to commercial breeds. On the other hand, Guayabal, Capanaparo and Cunaviche pigs were located in separated cluster, being the Cunaviche pigs the closest to the Iberian Peninsula pigs. It is conclude that there is subdivision and moderate differentiation between Venezuelan Creole pig populations, being some groups close to the commercial breeds and others to pigs from Iberian Peninsula.
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References
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