Conservation of ram semen at 5 ºC using medium supplemented with 0.5, 1.0 and 5.0 mM of reduced glutathione

  • Alfredo Trejo Cordova Universidad del Papaloapan
  • Ismael Abad Benítez Universidad del Papaloapan
  • Víctor Manuel Meza Villalvazo Universidad del Papaloapan
  • Carolina Antonia Estrada Universidad del Papaloapan
  • Abel Villa Mancera Benemérita Universidad Autónoma de Puebla


The experiment was carried out to determine the most recommendable concentration of reduced glutathione (GSH) to be used during refrigeration of ram semen at 5 °C. Each of a total of 12 ejaculates from two Pelibuey rams was divided into four aliquots corresponding to the GSH concentrations evaluated (0.0, 0.5, 1.0 and 5 mM), which were stored under refrigeration (5 °C) for 24 h. Evaluation of motility and viability was performed at 0, 1, 2 and 24 h of storage. At 24 h, sperm motility was also evaluated at 37 °C (reactivation). At 0, 1 and 2 h of storage there were no significant differences in sperm motility among the different concentrations of GSH. At 24 h, the concentration of 5 mM resulted in the highest (p <0.05) sperm motility (29.22%) and the highest (p <0.05) percentage of mobility during the reactivation (80.29%). As for viability, at 24 h of storage, the samples diluted with 5 mM GSH had 81.44% live spermatozoa. It is concluded that the activity of GSH as an antioxidant and protector during storage at 5 °C is dependent on concentration and storage time.


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Author Biographies

Alfredo Trejo Cordova, Universidad del Papaloapan
Universidad del Papaloapan, campus Loma Bonita, Oaxaca. México. Licenciatura en Zootecnia
Ismael Abad Benítez, Universidad del Papaloapan
Estudiante de la licenciatura en Zootecnia.
Víctor Manuel Meza Villalvazo, Universidad del Papaloapan
Reproducción Animal Asistida
Carolina Antonia Estrada, Universidad del Papaloapan
Abel Villa Mancera, Benemérita Universidad Autónoma de Puebla
Facultad de Medicina Veterinaria y Zootecnia. Área: Reproducción y Genética
How to Cite
Trejo Cordova, Alfredo, Ismael Abad Benítez, Víctor Manuel Meza Villalvazo, Carolina Antonia Estrada, and Abel Villa Mancera. 2017. “Conservation of Ram Semen at 5 ºC Using Medium Supplemented With 0.5, 1.0 and 5.0 MM of Reduced Glutathione”. Latin American Archives of Animal Production 25 (1-2).
Original paper